解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Trypsin-releasable glycosaminoglycans from the luminal surface of intact pig aorta were measured following metabolic labelling with [35S]sulphate. Chondroitin sulphate was found to be present at a surface density equal to that already established for heparan sulphate (5 X 10(11) chains per cm2). This result was confir...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01124788
更新日期:1987-03-01 00:00:00
abstract::Native bovine parathyroid hormone (bPTH) was found to be readily cleaved with human leukocyte elastase to yield the fragments bPTH(1-41) and bPTH(42-84). These were then isolated by reverse-phase HPLC and characterised by gas-phase sequencing and amino acid analysis. The biological activities of these fragments were a...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122726
更新日期:1987-01-01 00:00:00
abstract::The cDNA encoding about half of an antigenic non-surface schistosome parasite protein of Mr 97 K has recently been cloned and sequenced (Lanar, Pearce, James and Sher (1986) Science 234:593-596). Analysis of this sequence, together with the properties of the native protein, reveals that this protein is paramyosin, the...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122722
更新日期:1987-01-01 00:00:00
abstract::Bleaching of the purple membrane strongly reduces the number of divalent cation binding sites as well as their affinities. Conversely, deionization of the bleached membrane drastically inhibits the chromophore regeneration. Proteolysis experiments using bromelain show that the bleached membrane has an additional cleav...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114972
更新日期:1986-11-01 00:00:00
abstract::The sodium contents of beta-cell-rich pancreatic islets from ob/ob-mice were measured with an integrating flame photometer. After washing to an apparent steady state with different types of ice-cold media, islets incubated in the absence of glucose contained 79-108 mmol sodium kg-1 dry weight. Exposure to glucose resu...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114973
更新日期:1986-11-01 00:00:00
abstract::The kinetics of the fusion process between erythrocyte ghosts, as induced by Sendai virus, were readily revealed by a simple fluorescence procedure previously employed to characterize the fusion of viruses with biological membranes. The method relies on the relief of fluorescence selfquenching of the membrane-inserted...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114971
更新日期:1986-11-01 00:00:00
abstract::Sulphate efflux from human placental microvillus membrane vesicles was inhibited by external DIDS (KI congruent to 10(-6) M). This inhibition was partially reversed on addition of the translocated substrates sulphate or selenate to the external medium: selenite which is not translocated does not protect against DIDS i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116242
更新日期:1986-10-01 00:00:00
abstract::The solvation properties of ubiquinone-10 and ubiquinol-10 in a wide variety of solvents of polarity varying from alkanes to water are reported. Greatest solubility is observed in solvents of intermediate polarity and particularly where low polarity is combined with a pronounced tendency to interact with the benzoquin...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117101
更新日期:1986-09-01 00:00:00
abstract::Hypocaloric diet feeding reduced the mitochondrial protein content and whole tissue GDP-binding in interscapular brown adipose tissue from both virgin and lactating rats. A reduction in brown fat lipoprotein lipase activity was also detected in underfed virgin and lactating animals. These results indicate that lactati...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114762
更新日期:1986-07-01 00:00:00
abstract::Virus replication inhibitory peptide (carbobenzoxy-D-Phe-L-PheGly) was shown to be a potent specific inhibitor of the replication of paramyxovirus and myxovirus (Richardson, Scheid and Choppin (1980), Virology 105, 205-222). This peptide inhibits the membrane fusing activity of a viral glycoprotein. Many agents which ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114759
更新日期:1986-07-01 00:00:00
abstract::An antibody was raised to the synthetic pentapeptide pGluHisProGlyLys which, in radioimmunoassay (RIA), could detect the pentapeptide at a level of 10 fmole per tube and exhibited less than 0.5 per cent cross reactivity with a series of related peptides. The RIA was used to demonstrate the presence of C-terminally ext...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114948
更新日期:1986-06-01 00:00:00
abstract::The recognition of phosphate and sulphate esters of tyrosine residues has been studied employing antisera with specificity for tyrosine phosphate, and the enzymes aryl sulphatase, and acid and alkaline phosphatases. The ability of tyrosine phosphate, and of phosphate esters of phenol, to inhibit the antiserum was pH d...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01115155
更新日期:1986-03-01 00:00:00
abstract::Incubation of mouse thymocytes with 10 microM monensin for 1 hour induces morphological alterations characterized by the extensive dilatation and vacuolization of the Golgi complex. This effect is used to study the transport and utilization of labelled sugar nucleotides into intracellular vesicles by using thymocytes ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01115011
更新日期:1986-02-01 00:00:00
abstract::Poly(A)+RNA was isolated from brown adipose tissue of cold acclimated rats and a fraction enriched for uncoupling protein mRNA was used to generate a cDNA library in pBR 322. Immunological screening of 1,500 colonies with an affinity-purified antiserum against the uncoupling protein yielded five positive clones, pUCPr...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01145183
更新日期:1986-01-01 00:00:00
abstract::A non-ionic detergent such as Lubrol-PX extracts in soluble form the VIP-binding structures of rat liver plasma membranes. Detergent-solubilized proteins bind specifically [125I]VIP and the complex tracer-protein is identified by the use of Sepharose 6B columns. The interaction is only possible in the absence of deter...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01145177
更新日期:1986-01-01 00:00:00
abstract::Rabbit reticulocyte lysates, gel filtered on Sephadex G-25 with or without ATP (or its analogs), were preincubated at 37 degrees C and their subsequent binding to p3A4,3'-[32P]pCp was studied. Lysates filtered without ATP or in the presence of 0.1 mM 8-bromo-ATP, 1,N6-etheno-ATP, or ITP showed a time-dependent decreas...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119625
更新日期:1985-12-01 00:00:00
abstract::Rodney Porter's separation of antibody molecules into Fab and Fc fragments engendered the notion that a single antibody polypeptide chain might be coded by two or more genes. This concept profoundly influenced the development of molecular immunology over the past 25 years. Our current knowledge of antibody gene organi...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119901
更新日期:1985-10-01 00:00:00
abstract::This paper presents a short review of our contribution to the knowledge of the structure and function of human C1r, the activation unit of C1, the first component of the classical pathway of complement. On the basis of the domain structure of C1r, a model accounting for its autolytic activation mechanism is proposed. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119894
更新日期:1985-10-01 00:00:00
abstract::Cathepsin D occurs in two forms, a single polypeptide chain (Mr 44 000) and a non-covalent complex of two peptides of Mr 14 000 and 30 000 that is derived by proteolytic processing of the 44 000 polypeptide. The two forms from bovine spleen are closely similar in secondary structure content, in aromatic amino acid env...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119908
更新日期:1985-10-01 00:00:00
abstract::Decreasing extracellular sodium concentration was found to produce a contractile response of rabbit ileal smooth muscle. As the concentration decreases, the amplitude of contraction increases, thus producing a dose-dependent curve. Harmaline, a competitor for sodium, was found to inhibit the sodium gradient-dependent ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116998
更新日期:1985-08-01 00:00:00
abstract::An ontogenetic study of the effect of various neurohormones and other activators on adenylate cyclase systems was carried out using cultures of cells from 15-d-old embryonic mouse brain. Dopamine stimulated the enzyme activity at earlier culture ages (i.e. 4 and 10 d) but had little stimulatory effect at later ages (i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117073
更新日期:1985-07-01 00:00:00
abstract::Glycine methyl ester, an inhibitor of transglutaminase, decreased glucose-stimulated insulin release and delayed proinsulin conversion in rat pancreatic islets pulse-labelled with L-[4-3H]phenylalanine. Sarcosine methyl ester, which does not inhibit transglutaminase activity, failed to affect insulin release and proin...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117071
更新日期:1985-07-01 00:00:00
abstract::Human endothelial cells from the umbilical vein and skin fibroblasts can be separated by means of centrifugation in a density gradient of Percoll. Cells show a good recovery in culture. Viability is not impaired. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116944
更新日期:1985-06-01 00:00:00
abstract::Specific binding sites for somatostatin have been found in the cytosolic fractions of both parietal and non-parietal cells from rabbit gastric fundic mucosa. The stoichiometric data suggested the presence of two classes of binding sites in both types of cells. The number of low-affinity binding sites was significantly...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116904
更新日期:1985-04-01 00:00:00
abstract::The inhibition of passive K+ influx into human red blood cells (RBC) by loop diuretics was found to be dependent on the external Na+ concentration. In the absence of external Na+, there was minimal inhibition but the influx remained dependent on Cl- ions. Thus, raising the external Na+ concentration increased the affi...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116900
更新日期:1985-04-01 00:00:00
abstract::Pregnant rabbit mammary gland explants cultured with insulin, prolactin and cortisol synthesize and secrete transferrin radiolabelled with [3H]leucine or [3H]mannose. Addition of tunicamycin inhibits the glycosylation but not synthesis of the [3H]leucine-labelled polypeptide. Secretion of the non-glycosylated protein ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119592
更新日期:1985-03-01 00:00:00
abstract::Western blotting was used as a powerful alternative to immunoprecipitation for the detection of the simian virus 40 (SV40) large tumor (T) antigen. After resolution by electrophoresis on a SDS-polyacrylamide gel of a [35S]methionine labeled crude extract from SV40 infected monkey kidney cells, the separated proteins w...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117060
更新日期:1985-02-01 00:00:00
abstract::The sarcolemma of smooth-muscle cells from human pregnant myometrium possesses a high-affinity Ca2+-ATPase, which has the characteristics of an active Ca2+-extrusion pump. This pump enzyme, either membrane-bound or solubilized, was strongly inhibited by oxytocin (half-maximal inhibition at about 4 microU/ml or 10 pM)....
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117437
更新日期:1985-01-01 00:00:00
abstract::Perfusion of Langendorff rat hearts with [14C]adenosine yields an acid-insoluble, radioactive product whose concentration falls during ischaemia. The properties of the substance show that it is a polyribonucleotide. It is suggested that it may be mitochondrial poly A acting as a storage form of adenine nucleotides. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117435
更新日期:1985-01-01 00:00:00
abstract::Isolated mouse pancreatic islets were infected in vitro with two strains of Coxsackie B4 virus--a tissue culture-adapted strain and a mouse pancreas-adapted strain. Within 48 h of infection changes had occurred in the biochemical activities of islets infected with the mouse pancreas-adapted strain of virus. Basal insu...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117442
更新日期:1985-01-01 00:00:00
abstract::A possible role for Ca2+ and calmodulin in the action of growth-hormone-releasing factor (GHRF) was investigated. Low extracellular Ca2+ (less than 100 microM), methoxyverapamil, flunarizine, cinnarizine, and Co2+ decreased both basal and GHRF-stimulated growth-hormone secretion, but did not totally inhibit GHRF-stimu...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116691
更新日期:1984-12-01 00:00:00
abstract::Three myoid-cell clones were established from the thymuses of two Wistar rats; one thymus yielded two clones, R615A and R615B2, and the other yielded one clone, R613Ad. The three clones were divided into two subtypes. Both subtypes were able to form myofibrils, expressed AChR on their cell-surface membrane, and contai...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116890
更新日期:1984-11-01 00:00:00
abstract::The nucleotide sequence of the thioredoxin gene from Escherichia coli was determined. The structural gene was identified on a cloned 3-kb PvuII fragment by hybridization with a synthetic oligodeoxyribonucleotide corresponding to a part of the amino acid sequence of thioredoxin. Restriction-enzyme fragments were used a...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116889
更新日期:1984-11-01 00:00:00
abstract::In order to gain information on the metabolism of dolichol, the rates of excretion of dolichol and dolichyl phosphate were determined in rats maintained on a dolichol-free diet for 10 days. Analysis of fecal samples collected every 48 h yielded mean output values of 9.0 +/- 1.4 and 20.7 +/- 3.0 micrograms/rat/day of d...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01128818
更新日期:1984-09-01 00:00:00
abstract::The cellular onc-genes c-src and c-yes are expressed very differently during chicken embryonic development. The c-src mRNA and its translational product are detectable at high levels in brain extracts of chicken embryos and adult chickens, whereas muscle extracts show an age-dependent decrease in the amounts of c-src-...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01128817
更新日期:1984-09-01 00:00:00
abstract::The 250-kDa sialoglycoprotein of bovine erythrocyte membranes, GP-2, has been found to be an exceptionally rich source of branched sialo-oligosaccharides of poly-N-acetyllactosamine (I antigen) type with receptor activity for the human pathogen Mycoplasma pneumoniae. Desialylated GP-2 is the most potent I-active subst...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01128815
更新日期:1984-09-01 00:00:00
abstract::Calmodulin (CaM)-like activity was detected in human serum and foetal calf serum, with an activity 10 times more than that detectable in plasma. Serum CaM was largely accounted for by release from human platelets as confirmed by both radioimmunoassay and sodium-dodecyl-sulphate/polyacrylamide-gel electrophoresis of Ca...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121017
更新日期:1984-08-01 00:00:00
abstract::Sendai virus envelopes (SVE) were isolated from Sendai virus particles by Triton X-100 solubilization and ultracentrifugation. The envelopes were reconstituted in the presence of the fluorescent dye calcein by gradual removal of the detergent with Bio-beads SM-2. The internal volume of reconstituted Sendai virus envel...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121911
更新日期:1984-07-01 00:00:00
abstract::The effect of carbamylcholine and the calcium ionophore A23187 on catecholamine release and intracellular free calcium, [Ca2+]i, in bovine adrenal chromaffin cells was determined. At 10(-4) M carbamylcholine maximal release occurred with an accompanying increase in [Ca2+]i from a basal level of 168 nM to less than 300...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121918
更新日期:1984-07-01 00:00:00
abstract::Conflicting opinions were recently expressed concerning the possible effect of alpha 2-adrenergic agonists upon cyclic AMP production in pancreatic islets. In the present study, clonidine inhibited glucose-induced insulin release from rat pancreatic islets, this inhibitory effect being abolished by idazoxan. Clonidine...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122227
更新日期:1984-06-01 00:00:00